Microinjector

PM6000 4-Channel Programmable Injector

  • Complete capabilities for microinjection and perfusion
  • Up to four inject/perfusion outputs, one hold cell output and one synchronized drain out channel
  • Programmable and savable timers, counters and step sequences
  • Simultaneously real-time displays adjusted pressures, count and time
  • Convenient manual control or automatic sequential step cycle operation

The PM6000 Programmable 4-Channel Pressure Injector is designed for intracellular injection and extracellular perfusion. It can be used for directly injecting different molecular agents, labelled dyes, genes, RNA, DNA, proteins and cell organelles to a cell, or performing a perfusion of up to 15 combinations from four different agents and drugs to a cell or a group of cells. It is also a superior device for intracytoplasmic sperm injection and cell electrophysiology. By combining an advanced microcontroller and precise pneumatic components, the PM6000 can simultaneously control four injection micropipettes, one cell holding pipette and one drain pipette.

The four injection micropipettes can be controlled separately or combined together to perform actions such as injection, capillary action balancing, suction or clear up. The cell holding pipette can produce an adjusted vacuum to hold a cell or eject a pressure to push a cell or just clear the holding pipette itself. Also, the drain pipette can produce an adjusted vacuum to drain out liquid precisely and synchronize with every microperfusion. The PM6000 can deliver different agents and drugs from picolitters to continued perfusion.

There are individual regulators for adjustments of suction vacuum, cell holding vacuum, drain vacuum, inject pressure and balance pressure. A LCD window displays full information such as pressure, vacuum, time, sequence steps and actions.

During pressure mode, all pressure readings are simultaneously real-time displayed on the LCD screen so that the user can conveniently preset all the pressures without switching the pressure measurement. Meanwhile, previous pressure reading can be recovered on the LCD for further comparison.

The PM6000 provides programmable timers and counters to deliver accurate timing and counting for corresponding functions.

The PM6000 allows the user to program custom-designed action sequences. There are 16 savable sequences with 32 programmable steps in each sequence. The sequences can be used to perform and repeat different injections and perfusion cycles precisely. Also, the sequential cycles can be automatically performed or triggered each step by manual. Even the interval time between steps is programmable for automatic sequential cycle.

Not only all preset timers, counters and sequential steps can be saved when the power is turn off, the pressure readings can also be saved by a push of a button. Therefore, the PM6000 can reproduce experiments at anytime completely.

An attached foot switch can control all preset step or be used as an injection trigger. An optional remote keyboard gives the user another convenient tool to control the whole operation. The exceptional versatility and extremely precise control are the hallmark of the PM6000.

 


Specifications

Output Channels Eight injection / perfusion outputs, one synchronized drain out and one cell hold channel
Input Gas Pressure 30 - 100 psi
Clearing Pressure Same as input pressure
Clear Hold Pressure 0-3 psi (regulated)
Injection Pressure 0.05 - 60 psi ( regulated )
Balance Pressure 0.05 - 10 psi ( regulated )
Fill Vacuum 0" - 24" Hg ( regulated )
Hold Vacuum 0" - 30" of water (regulated )
Drain Vacuum 0" - 30" of water ( regulated )
Repeatability +/- 0.02 psi
Display Accuracy +/- 0.05 psi for pressure, +/- 0.2" for vacuum (<2" H2O).
Timer Setting Range 0 msec - 327.67 sec. (10 msec. Resolution for whole range).
Counter Setting Range 1 - 255
Savable Sequences 16 sequences
Programmable Steps 32 steps for each sequence.
Remote Control Ports DB9 connector for remote key pad, foot switch jack and BNC for negative TTL trigger, a jack connector for injection foot switch.
Signal Output Ports Eight BNCs for injection signal on output port 1-4.
Power Consumption 100, 120 or 220 VAC, 35 Watts